Anti-14-3-3 gamma Rabbit Monoclonal Antibody - 产品中心

Anti-14-3-3 gamma Rabbit Monoclonal Antibody #4226

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Flow cytometric analysis of 14-3-3 gamma expression in HepG2 cells using anti-14-3-3 gamma antibody (Cat#4226, 1:2,000). Green, isotype control; red, 14-3-3 gamma.

Immunocytochemical staining of HepG2 cells with anti-14-3-3 gamma antibody (Cat#4226, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-14-3-3 gamma antibody (Cat#4226). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 gamma antibody (Cat#4226, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

YWHAG
货号：

4226
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-14-3-3 gamma Rabbit Monoclonal Antibody
Aliases	YWHAG; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Gamma; 14-3-3GAMMA; PPP1R170; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Gamma Polypeptide; Protein Phosphatase 1, Regulatory Subunit 170; Protein Kinase C Inhibitor Protein 1; 14-3-3 Protein Gamma; KCIP-1; 14-3-3 Gamma; 14-3-3γ; 14-3-3G; EIEE56; DEE56
Background	Gene Name: YWHAG NCBI Gene Entry: 7532 UniProt Entry: P61981
Application Information	Molecular Weight: Predicted, 28 kDa; observed, 28 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10165 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human 14-3-3 gamma
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of 14-3-3 gamma expression in HepG2 cells using anti-14-3-3 gamma antibody (Cat#4226, 1:2,000). Green, isotype control; red, 14-3-3 gamma.
Immunocytochemical staining of HepG2 cells with anti-14-3-3 gamma antibody (Cat#4226, 1:1,000). Nuclei were stained blue with DAPI; 14-3-3 gamma was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-14-3-3 gamma antibody (Cat#4226). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-14-3-3 gamma antibody (Cat#4226, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

YWHAG
货号：

4226
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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