Anti-SMAD Family Member 1 Rabbit Monoclonal Antibody - 产品中心

Anti-SMAD Family Member 1 Rabbit Monoclonal Antibody #4231

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Flow cytometric analysis of SMAD1 expression in HAP-1 cells using anti-SMAD1 antibody (Cat#4231, 1:2,000). Green, isotype control; red, SMAD1.

Immunocytochemical staining of HAP-1 cells with anti-SMAD1 antibody (Cat#4231, 1:1,000). Nuclei were stained blue with DAPI; SMAD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-SMAD1 antibody (Cat#4231). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMAD1 antibody (Cat#4231, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

SMAD1
货号：

4231
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-SMAD Family Member 1 Rabbit Monoclonal Antibody
Aliases	SMAD1; SMAD Family Member 1; MADR1; JV4-1; MADH1; Mothers Against Decapentaplegic Homolog 1; Mothers Against DPP Homolog 1; Mad-Related Protein 1; BSP-1; BSP1; MAD, Mothers Against Decapentaplegic Homolog 1 (Drosophila); Transforming Growth Factor-Beta Signaling Protein 1; Transforming Growth Factor-Beta-Signaling Protein 1; SMAD, Mothers Against DPP Homolog 1 (Drosophila); MAD, Mothers Against Decapentaplegic Homolog 1; SMAD, Mothers Against DPP Homolog 1; TGF-Beta Signaling Protein 1; MAD Homolog 1; SMAD 1; HSMAD1; Smad1; JV41
Background	Gene Name: SMAD1 NCBI Gene Entry: 4086 UniProt Entry: Q15797
Application Information	Molecular Weight: Predicted, 52 kDa; observed, 58 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10190 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Smad1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of SMAD1 expression in HAP-1 cells using anti-SMAD1 antibody (Cat#4231, 1:2,000). Green, isotype control; red, SMAD1.
Immunocytochemical staining of HAP-1 cells with anti-SMAD1 antibody (Cat#4231, 1:1,000). Nuclei were stained blue with DAPI; SMAD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-SMAD1 antibody (Cat#4231). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMAD1 antibody (Cat#4231, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

SMAD1
货号：

4231
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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