Flow cytometric analysis of phospho-AKT1 (S124) expression in C2C12 cells using anti-phospho-AKT1 (S124) antibody (Cat#4250, 1:2,000). Green, isotype control; red, phospho-AKT1 (S124).
Immunocytochemical staining of C2C12 cells with anti-Phospho-AKT1 (S124) antibody (Cat#4250, 1:1,000). Nuclei were stained blue with DAPI; Phospho-AKT1 (S124) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-AKT1 (S124) antibody (Cat#4250). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-AKT1 (S124) antibody (Cat#4250, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of phospho-AKT1 (S124) expression in C2C12 cells using anti-phospho-AKT1 (S124) antibody (Cat#4250, 1:2,000). Green, isotype control; red, phospho-AKT1 (S124).
Immunocytochemical staining of C2C12 cells with anti-Phospho-AKT1 (S124) antibody (Cat#4250, 1:1,000). Nuclei were stained blue with DAPI; Phospho-AKT1 (S124) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-AKT1 (S124) antibody (Cat#4250). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-AKT1 (S124) antibody (Cat#4250, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of phospho-AKT1 (S124) expression in C2C12 cells using anti-phospho-AKT1 (S124) antibody (Cat#4250, 1:2,000). Green, isotype control; red, phospho-AKT1 (S124).
Immunocytochemical staining of C2C12 cells with anti-Phospho-AKT1 (S124) antibody (Cat#4250, 1:1,000). Nuclei were stained blue with DAPI; Phospho-AKT1 (S124) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-AKT1 (S124) antibody (Cat#4250). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-AKT1 (S124) antibody (Cat#4250, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
