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Anti-KMT6 / EZH2 Rabbit Rabbit Monoclonal Antibody #4283

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Flow cytometric analysis of KMT6/EZH2 expression in HepG2 cells using anti-KMT6/EZH2 antibody (Cat#4283, 1:2,000). Green, isotype control; red, KMT6/EZH2.
Immunocytochemical staining of HepG2 cells with anti-KMT6 / EZH2 antibody (Cat#4283, 1:1,000) . Nuclei were stained blue with DAPI; KMT6 / EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-KMT6/EZH2 antibody (Cat#4283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KMT6/EZH2 antibody (Cat#4283, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    EZH2
  • 货号:
    4283
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-KMT6 / EZH2 Rabbit Rabbit Monoclonal Antibody
Aliases EZH2; Enhancer Of Zeste 2 Polycomb Repressive Complex 2 Subunit; ENX-1; KMT6; KMT6A; Histone-Lysine N-Methyltransferase EZH2; Lysine N-Methyltransferase 6; Enhancer Of Zeste Homolog 2; EZH1; Enhancer Of Zeste (Drosophila) Homolog 2; Enhancer Of Zeste Homolog 2 (Drosophila); EC 2.1.1.356; EC 2.1.1.43; EC 2.1.1; EZH2b; ENX1; WVS2; WVS
Background

Gene Name: EZH2

NCBI Gene Entry: 2146

UniProt Entry: Q15910

Application Information

Molecular Weight: Predicted, 85 kDa; observed, 98 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10445

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human KMT6
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of KMT6/EZH2 expression in HepG2 cells using anti-KMT6/EZH2 antibody (Cat#4283, 1:2,000). Green, isotype control; red, KMT6/EZH2.
  • Immunocytochemical staining of HepG2 cells with anti-KMT6 / EZH2 antibody (Cat#4283, 1:1,000) . Nuclei were stained blue with DAPI; KMT6 / EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-KMT6/EZH2 antibody (Cat#4283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KMT6/EZH2 antibody (Cat#4283, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of KMT6/EZH2 expression in HepG2 cells using anti-KMT6/EZH2 antibody (Cat#4283, 1:2,000). Green, isotype control; red, KMT6/EZH2.
Immunocytochemical staining of HepG2 cells with anti-KMT6 / EZH2 antibody (Cat#4283, 1:1,000) . Nuclei were stained blue with DAPI; KMT6 / EZH2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-KMT6/EZH2 antibody (Cat#4283). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-KMT6/EZH2 antibody (Cat#4283, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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