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Anti-UCHL1 Rabbit Monoclonal Antibody #4287

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Flow cytometric analysis of UCHL1 expression in HAP-1 cells using anti-UCHL1 antibody (Cat#4287, 1:2,000). Green, isotype control; red, UCHL1.

Immunocytochemical staining of HAP-1 cells with anti-UCHL1 antibody (Cat#4287, 1:1,000). Nuclei were stained blue with DAPI; UCHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-UCHL1 antibody (Cat#4287). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UCHL1 antibody (Cat#4287, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

UCHL1
货号：

4287
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-UCHL1 Rabbit Monoclonal Antibody
Aliases	UCHL1; Ubiquitin C-Terminal Hydrolase L1; PGP9.5; UCHL-1; Ubiquitin Carboxyl-Terminal Esterase L1 (Ubiquitin Thiolesterase); Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L1; Neuron Cytoplasmic Protein 9.5; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase; PGP 9.5; Uch-L1; UCH-L1; PARK5; Epididymis Secretory Protein Li 53; Epididymis Luminal Protein 117; EC 3.4.19.12; HEL-S-53; HEL-117; SPG79A; NDGOA; PGP95; SPG79
Background	Gene Name: UCHL1 NCBI Gene Entry: 7345 UniProt Entry: P09936
Application Information	Molecular Weight: Predicted, 25 kDa; observed, 25 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10465 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PGP9.5
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of UCHL1 expression in HAP-1 cells using anti-UCHL1 antibody (Cat#4287, 1:2,000). Green, isotype control; red, UCHL1.
Immunocytochemical staining of HAP-1 cells with anti-UCHL1 antibody (Cat#4287, 1:1,000). Nuclei were stained blue with DAPI; UCHL1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-UCHL1 antibody (Cat#4287). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UCHL1 antibody (Cat#4287, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

UCHL1
货号：

4287
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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