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Anti-ATP2A2 Rabbit Monoclonal Antibody #4296

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Flow cytometric analysis of ATP2A2 expression in HeLa cells using anti-ATP2A2 antibody (Cat#4296, 1:2,000). Green, isotype control; red, ATP2A2.

Immunocytochemical staining of Hela cells with anti-ATP2A2 antibody (Cat#4296, 1:1,000). Nuclei were stained blue with DAPI; ATP2A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ATP2A2 antibody (Cat#4296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP2A2 antibody (Cat#4296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ATP2A2
货号：

4296
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-ATP2A2 Rabbit Monoclonal Antibody
Aliases	ATP2A2; ATPase Sarcoplasmic/Endoplasmic Reticulum Ca2+ Transporting 2; SERCA2; Sarcoplasmic/Endoplasmic Reticulum Calcium ATPase 2; Calcium Pump 2; ATP2B; Endoplasmic Reticulum Class 1/2 Ca(2+) ATPase; SR Ca(2+)-ATPase; DAR; Calcium-Transporting ATPase Sarcoplasmic Reticulum Type, Slow Twitch Skeletal Muscle Isoform; ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2; ATPase Ca++ Transporting Cardiac Muscle Slow Twitch 2; ATPase, Ca++ Dependent, Slow-Twitch, Cardiac Muscle-2; Cardiac Ca2+ ATPase; EC 7.2.2.10; EC 3.6.3.8; EC 3.6.3; DD
Background	Gene Name: ATP2A2 NCBI Gene Entry: 488 UniProt Entry: P16615
Application Information	Molecular Weight: Predicted, 115 kDa; observed, 110 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10510 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human SERCA2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATP2A2 expression in HeLa cells using anti-ATP2A2 antibody (Cat#4296, 1:2,000). Green, isotype control; red, ATP2A2.
Immunocytochemical staining of Hela cells with anti-ATP2A2 antibody (Cat#4296, 1:1,000). Nuclei were stained blue with DAPI; ATP2A2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATP2A2 antibody (Cat#4296). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP2A2 antibody (Cat#4296, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ATP2A2
货号：

4296
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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