Flow cytometric analysis of annexin V expression in HT-1080 cells using anti-annexin V antibody (Cat#4304, 1:2,000). Green, isotype control; red, annexin V.
Immunocytochemical staining of HT-1080 cells with anti-ANXA5 antibody(Cat#4304, 1:1,000). Nuclei were stained blue with DAPI; ANXA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-annexin V antibody (Cat#4304). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin V antibody (Cat#4304, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of annexin V expression in HT-1080 cells using anti-annexin V antibody (Cat#4304, 1:2,000). Green, isotype control; red, annexin V.
Immunocytochemical staining of HT-1080 cells with anti-ANXA5 antibody(Cat#4304, 1:1,000). Nuclei were stained blue with DAPI; ANXA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-annexin V antibody (Cat#4304). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin V antibody (Cat#4304, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of annexin V expression in HT-1080 cells using anti-annexin V antibody (Cat#4304, 1:2,000). Green, isotype control; red, annexin V.
Immunocytochemical staining of HT-1080 cells with anti-ANXA5 antibody(Cat#4304, 1:1,000). Nuclei were stained blue with DAPI; ANXA5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-annexin V antibody (Cat#4304). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-annexin V antibody (Cat#4304, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
