Anti-Poly(A) Binding Protein Nuclear 1 Rabbit Monoclonal Antibody - 产品中心

Anti-Poly(A) Binding Protein Nuclear 1 Rabbit Monoclonal Antibody #4317

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Flow cytometric analysis of PABPN1 expression in HepG2 cells using anti-PABPN1 antibody (Cat#4317, 1:2,000). Green, isotype control; red, PABPN1.

Immunocytochemical staining of Hela cells with anti-PABPN1 antibody (Cat#4317, 1:1,000) . Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-PABPN1 antibody (Cat#4317). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PABPN1 antibody (Cat#4317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PABPN1
货号：

4317
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Poly(A) Binding Protein Nuclear 1 Rabbit Monoclonal Antibody
Aliases	Poly(A) Binding Protein Nuclear 1; PAB2 ; PABP2; Polyadenylate-Binding Protein 2; PABP-2; PABII; OPMD; Polyadenylate-Binding Nuclear Protein 1; Poly(A)-Binding Protein, Nuclear 1; Poly(A) Binding Protein, Nuclear 1; Nuclear Poly(A)-Binding Protein 1; Poly(A) Binding Protein II; Poly(A)-Binding Protein II; Poly(A) Binding Protein 2; Poly(A)-Binding Protein 2
Background	Gene Name: PABPN1 NCBI Gene Entry: 8106 UniProt Entry: Q86U42
Application Information	Molecular Weight: Predicted, 33 kDa; observed, 49 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10615 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PABPN1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PABPN1 expression in HepG2 cells using anti-PABPN1 antibody (Cat#4317, 1:2,000). Green, isotype control; red, PABPN1.
Immunocytochemical staining of Hela cells with anti-PABPN1 antibody (Cat#4317, 1:1,000) . Nuclei were stained blue with DAPI; PABPN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-PABPN1 antibody (Cat#4317). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PABPN1 antibody (Cat#4317, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PABPN1
货号：

4317
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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