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Anti-Ku70 Rabbit Monoclonal Antibody #4318

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Flow cytometric analysis of Ku70 expression in HepG2 cells using anti-Ku70 antibody (Cat#4318, 1:2,000). Green, isotype control; red, Ku70.
Immunocytochemical staining of HepG2 cells with anti-Ku70 antibody (Cat#4318, 1:1,000). Nuclei were stained blue with DAPI; Ku70 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-ku70 antibody (Cat#4318). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ku70 antibody (Cat#4318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    XRCC6
  • 货号:
    4318
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Ku70 Rabbit Monoclonal Antibody
Aliases XRCC6; X-Ray Repair Cross Complementing 6; X-Ray Repair Complementing Defective Repair In Chinese Hamster Cells 6; G22P1; KU70; ML8; X-Ray Repair Cross-Complementing Protein 6; Thyroid Autoantigen 70kDa (Ku Antigen); ATP-Dependent DNA Helicase 2 Subunit 1; Thyroid Autoantigen 70kD (Ku Antigen); 5'-Deoxyribose-5-Phosphate Lyase Ku70; Lupus Ku Autoantigen Protein P70; 70 KDa Subunit Of Ku Antigen; DNA Repair Protein XRCC6; Ku Autoantigen, 70kDa; 5'-DRP Lyase Ku70; D22S731; D22S671; CTC75; CTCBF; TLAA; ATP-Dependent DNA Helicase II, 70 KDa Subunit; ATP-Dependent DNA Helicase II 70 KDa Subunit; CTC Box Binding Factor 75 KDa Subunit; CTC Box-Binding Factor 75 KDa Subunit; Thyroid-Lupus Autoantigen P70; Ku Autoantigen P70 Subunit; Thyroid-Lupus Autoantigen; EC 4.2.99.-; EC 3.6.4.-; Ku70
Background

Gene Name: XRCC6

NCBI Gene Entry: 2547

UniProt Entry: P12956

Application Information

Molecular Weight: Predicted, 70 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10620

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Ku70
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Ku70 expression in HepG2 cells using anti-Ku70 antibody (Cat#4318, 1:2,000). Green, isotype control; red, Ku70.
  • Immunocytochemical staining of HepG2 cells with anti-Ku70 antibody (Cat#4318, 1:1,000). Nuclei were stained blue with DAPI; Ku70 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-ku70 antibody (Cat#4318). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ku70 antibody (Cat#4318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Ku70 expression in HepG2 cells using anti-Ku70 antibody (Cat#4318, 1:2,000). Green, isotype control; red, Ku70.
Immunocytochemical staining of HepG2 cells with anti-Ku70 antibody (Cat#4318, 1:1,000). Nuclei were stained blue with DAPI; Ku70 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-ku70 antibody (Cat#4318). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ku70 antibody (Cat#4318, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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