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Anti-Beta-secretase 1 Rabbit Monoclonal Antibody #4326

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Flow cytometric analysis of Beta-secretase 1 expression in C2C12 cells using anti-Beta-secretase 1 antibody (Cat#4326, 1:2,000). Green, isotype control; red, Beta-secretase 1.
Immunocytochemical staining of C2C12 cells with anti-Beta-secretase 1 antibody (Cat#4326, 1:1,000). Nuclei were stained blue with DAPI; Beta-secretase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-beta-secretase 1 antibody (Cat#4326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta-secretase 1 antibody (Cat#4326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    BACE1
  • 货号:
    4326
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Beta-secretase 1 Rabbit Monoclonal Antibody
Aliases BACE1; Beta-Secretase 1; BACE; Membrane-Associated Aspartic Protease; Beta-Site APP Cleaving Enzyme 1; Beta-Site APP-Cleaving Enzyme
Aspartyl Protease; EC 3.4.23.46; Memapsin-2; Asp 2; ASP2; Beta-Site Amyloid Beta A4 Precursor Protein-Cleaving Enzyme; Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1; Transmembrane Aspartic Proteinase Asp2; Beta-Site APP-Cleaving Enzyme 1; APP Beta-Secretase; EC 3.4.23; KIAA1149; HSPC104
Background

Gene Name: BACE1

NCBI Gene Entry: 23621

UniProt Entry: P56817

Application Information

Molecular Weight: Predicted, 56 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10660

Species Reactivity: Mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human BACE1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Beta-secretase 1 expression in C2C12 cells using anti-Beta-secretase 1 antibody (Cat#4326, 1:2,000). Green, isotype control; red, Beta-secretase 1.
  • Immunocytochemical staining of C2C12 cells with anti-Beta-secretase 1 antibody (Cat#4326, 1:1,000). Nuclei were stained blue with DAPI; Beta-secretase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-beta-secretase 1 antibody (Cat#4326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta-secretase 1 antibody (Cat#4326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of Beta-secretase 1 expression in C2C12 cells using anti-Beta-secretase 1 antibody (Cat#4326, 1:2,000). Green, isotype control; red, Beta-secretase 1.
Immunocytochemical staining of C2C12 cells with anti-Beta-secretase 1 antibody (Cat#4326, 1:1,000). Nuclei were stained blue with DAPI; Beta-secretase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-beta-secretase 1 antibody (Cat#4326). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta-secretase 1 antibody (Cat#4326, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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