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Anti-ATG4B Rabbit Monoclonal Antibody #4335

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Flow cytometric analysis of ATG4B expression in C2C12 cells using anti-ATG4B antibody (Cat#4335, 1:2,000). Green, isotype control; red, ATG4B.

Immunocytochemical staining of C2C12 cells with anti-ATG4B antibody (Cat#4335, 1:1,000) . Nuclei were stained blue with DAPI; ATG4B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-ATG4B antibody (Cat#4335). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4B antibody (Cat#4335, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ATG4B
货号：

4335
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-ATG4B Rabbit Monoclonal Antibody
Aliases	ATG4B; Autophagy Related 4B Cysteine Peptidase; AUTL1; KIAA0943; APG4B; Autophagy-Related Cysteine Endopeptidase 1; Autophagy-Related Protein 4 Homolog B; AUT-Like 1 Cysteine Endopeptidase; Cysteine Protease ATG4B; Autophagin-1; HsAPG4B; HAPG4B; ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae); APG4 Autophagy 4 Homolog B (S. Cerevisiae); Autophagy Related 4B, Cysteine Peptidase; ATG4 Autophagy Related 4 Homolog B; APG4 Autophagy 4 Homolog B; DKFZp586D1822; DKFZP586D1822; EC 3.4.22.-; EC 3.4.22; Apg4B
Background	Gene Name: ATG4B NCBI Gene Entry: 23192 UniProt Entry: Q9Y4P1
Application Information	Molecular Weight: Predicted, 44 kDa; observed, 44 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB10705 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Atg4B
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATG4B expression in C2C12 cells using anti-ATG4B antibody (Cat#4335, 1:2,000). Green, isotype control; red, ATG4B.
Immunocytochemical staining of C2C12 cells with anti-ATG4B antibody (Cat#4335, 1:1,000) . Nuclei were stained blue with DAPI; ATG4B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-ATG4B antibody (Cat#4335). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4B antibody (Cat#4335, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ATG4B
货号：

4335
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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