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Anti-Atg4C Rabbit Monoclonal Antibody #4336

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Flow cytometric analysis of Atg4C expression in HepG2 cells using anti-Atg4C antibody (Cat#4336, 1:2,000). Green, isotype control; red, Atg4C.
Immunocytochemical staining of HepG2 cells with anti-Atg4C antibody (Cat#4336, 1:1,000). Nuclei were stained blue with DAPI; Atg4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATG4C antibody (Cat#4336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4C antibody (Cat#4336, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    ATG4C
  • 货号：
    4336
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Atg4C Rabbit Monoclonal Antibody
Aliases ATG4C; Autophagy Related 4C Cysteine Peptidase; AUTL3; APG4C; AUTL1; Autophagy-Related Cysteine Endopeptidase 3; Autophagy-Related Protein 4 Homolog C; AUT-Like 3 Cysteine Endopeptidase; Cysteine Protease ATG4C; Autophagin-3; FLJ14867; HsAPG4C; AUT (S. Cerevisiae)-Like 1, Cysteine Endopeptidase; AUT-Like 1, Cysteine Endopeptidase (S. Cerevisiae); ATG4 Autophagy Related 4 Homolog C (S. Cerevisiae); APG4 Autophagy 4 Homolog C (S. Cerevisiae); Epididymis Secretory Sperm Binding Protein; Autophagy Related 4C, Cysteine Peptidase; ATG4 Autophagy Related 4 Homolog C; AUT-Like 1, Cysteine Endopeptidase; APG4 Autophagy 4 Homolog C; EC 3.4.22.-; EC 3.4.22; APG4-C
Background

Gene Name: ATG4C

NCBI Gene Entry: 84938

UniProt Entry: Q96DT6
Application Information

Molecular Weight: Predicted, 52 kDa; observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB10710

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ATG4C
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Atg4C expression in HepG2 cells using anti-Atg4C antibody (Cat#4336, 1:2,000). Green, isotype control; red, Atg4C.
  • Immunocytochemical staining of HepG2 cells with anti-Atg4C antibody (Cat#4336, 1:1,000). Nuclei were stained blue with DAPI; Atg4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-ATG4C antibody (Cat#4336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4C antibody (Cat#4336, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    ATG4C
  • 货号：
    4336
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Atg4C expression in HepG2 cells using anti-Atg4C antibody (Cat#4336, 1:2,000). Green, isotype control; red, Atg4C.
Immunocytochemical staining of HepG2 cells with anti-Atg4C antibody (Cat#4336, 1:1,000). Nuclei were stained blue with DAPI; Atg4C was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATG4C antibody (Cat#4336). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATG4C antibody (Cat#4336, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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