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Anti-LMAN1 Rabbit Monoclonal Antibody #4405

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Flow cytometric analysis of LMAN1 expression in HepG2 cells using anti-LMAN1 antibody (Cat#4405, 1:2,000). Green, isotype control; red, LMAN1.

Immunocytochemical staining of HepG2 cells with anti-LMAN1 antibody (Cat#4405, 1:1,000). Nuclei were stained blue with DAPI; LMAN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-LMAN1 antibody (Cat#4405). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LMAN1 antibody (Cat#4405, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

LMAN1
货号：

4405
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-LMAN1 Rabbit Monoclonal Antibody
Aliases	LMAN1; Lectin, Mannose Binding 1; ERGIC53; ERGIC-53; MCFD1; FMFD1; F5F8D; MR60; Gp58; Endoplasmic Reticulum-Golgi Intermediate Compartment Protein 53; ER-Golgi Intermediate Compartment 53 KDa Protein; Intracellular Mannose-Specific Lectin MR60; Protein ERGIC-53; Coagulation Factor V-Factor VIII Combined Deficiency; Lectin, Mannose-Binding, 1; Lectin Mannose-Binding 1; GP58
Background	Gene Name: LMAN1 NCBI Gene Entry: 3998 UniProt Entry: P49257
Application Information	Molecular Weight: Predicted, 58 kDa; observed, 53 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11055 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human LMAN1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of LMAN1 expression in HepG2 cells using anti-LMAN1 antibody (Cat#4405, 1:2,000). Green, isotype control; red, LMAN1.
Immunocytochemical staining of HepG2 cells with anti-LMAN1 antibody (Cat#4405, 1:1,000). Nuclei were stained blue with DAPI; LMAN1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-LMAN1 antibody (Cat#4405). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LMAN1 antibody (Cat#4405, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

LMAN1
货号：

4405
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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