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Anti-Peptidase D Rabbit Monoclonal Antibody #4431

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Flow cytometric analysis of Peptidase D expression in HT-1080 cells using anti-Peptidase D antibody (Cat#4431, 1:2,000). Green, isotype control; red, Peptidase D.
Immunocytochemical staining of HT-1080 cells with anti-Peptidase D antibody (Cat#4431, 1:1,000). Nuclei were stained blue with DAPI; Peptidase D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-peptidase D antibody (Cat#4431). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-peptidase D antibody (Cat#4431, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    PEPD
  • 货号：
    4431
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-Peptidase D Rabbit Monoclonal Antibody
Aliases PEPD; Peptidase D; Xaa-Pro Dipeptidase; Imidodipeptidase; Proline Dipeptidase; X-Pro Dipeptidase; Prolidase; Testicular Tissue Protein Li 138; Aminoacyl-L-Proline Hydrolase; EC 3.4.13.9; PROLIDASE; PRD
Background

Gene Name: PEPD

NCBI Gene Entry: 5184

UniProt Entry: P12955
Application Information

Molecular Weight: Predicted, 55 kDa; observed, 55 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11185

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human PRD
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Peptidase D expression in HT-1080 cells using anti-Peptidase D antibody (Cat#4431, 1:2,000). Green, isotype control; red, Peptidase D.
  • Immunocytochemical staining of HT-1080 cells with anti-Peptidase D antibody (Cat#4431, 1:1,000). Nuclei were stained blue with DAPI; Peptidase D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-peptidase D antibody (Cat#4431). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-peptidase D antibody (Cat#4431, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    PEPD
  • 货号：
    4431
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of Peptidase D expression in HT-1080 cells using anti-Peptidase D antibody (Cat#4431, 1:2,000). Green, isotype control; red, Peptidase D.
Immunocytochemical staining of HT-1080 cells with anti-Peptidase D antibody (Cat#4431, 1:1,000). Nuclei were stained blue with DAPI; Peptidase D was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-peptidase D antibody (Cat#4431). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-peptidase D antibody (Cat#4431, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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