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Anti-PPP2R5E Rabbit Monoclonal Antibody #4442

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Flow cytometric analysis of PPP2R5E expression in HepG2 cells using anti-PPP2R5E antibody (Cat#4442, 1:2,000). Green, isotype control; red, PPP2R5E.
Immunocytochemical staining of HepG2 cells with anti-PPP2R5E antibody (Cat#4442, 1:1,000). Nuclei were stained blue with DAPI; PPP2R5E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PPP2R5E antibody (Cat#4442). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R5E antibody (Cat#4442, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    PPP2R5E
  • 货号：
    4442
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-PPP2R5E Rabbit Monoclonal Antibody
Aliases Protein Phosphatase 2 Regulatory Subunit B'Epsilon; B56epsilon; B56E; Serine/Threonine-Protein Phosphatase 2A 56 KDa Regulatory Subunit Epsilon Isoform; Serine/Threonine Protein Phosphatase 2A, 56 KDa Regulatory Subunit, Epsilon; Protein Phosphatase 2, Regulatory Subunit B (B56), Epsilon Isoform; Protein Phosphatase 2 Regulatory Subunit B', Epsilon; PP2A B Subunit Isoform PR61-Epsilon; PP2A B Subunit Isoform B56-Epsilon; PP2A B Subunit Isoform B'-Epsilon; PP2A B Subunit Isoform R5-Epsilon; PP2A, B Subunit, PR61 Epsilon; PP2A, B Subunit, B56 Epsilon; PP2A, B Subunit, B' Epsilon; PP2A, B Subunit, R5 Epsilon
Background

Gene Name: PPP2R5E

NCBI Gene Entry: 5529

UniProt Entry: Q16537
Application Information

Molecular Weight: Predicted, 55 kDa; observed, 50 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11240

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human PPP2R5E
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PPP2R5E expression in HepG2 cells using anti-PPP2R5E antibody (Cat#4442, 1:2,000). Green, isotype control; red, PPP2R5E.
  • Immunocytochemical staining of HepG2 cells with anti-PPP2R5E antibody (Cat#4442, 1:1,000). Nuclei were stained blue with DAPI; PPP2R5E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-PPP2R5E antibody (Cat#4442). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R5E antibody (Cat#4442, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    PPP2R5E
  • 货号：
    4442
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of PPP2R5E expression in HepG2 cells using anti-PPP2R5E antibody (Cat#4442, 1:2,000). Green, isotype control; red, PPP2R5E.
Immunocytochemical staining of HepG2 cells with anti-PPP2R5E antibody (Cat#4442, 1:1,000). Nuclei were stained blue with DAPI; PPP2R5E was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PPP2R5E antibody (Cat#4442). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PPP2R5E antibody (Cat#4442, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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