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Anti-Galactosidase alpha Rabbit Monoclonal Antibody #4459

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Flow cytometric analysis of galactosidase alpha expression in Hela cells using anti-galactosidase alpha antibody (Cat#4459, 1:2,000). Green, isotype control; red, galactosidase alpha.

Immunocytochemical staining of Hela cells with anti-Galactosidase alpha antibody (Cat#4459, 1:1,000). Nuclei were stained blue with DAPI; Galactosidase alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-galactosidase alpha antibody (Cat#4459). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-galactosidase alpha antibody (Cat#4459, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

GLA
货号：

4459
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-Galactosidase alpha Rabbit Monoclonal Antibody
Aliases	GLA; Galactosidase Alpha; GALA; Galactosylgalactosylglucosylceramidase GLA; Alpha-D-Galactosidase A; Alpha-Galactosidase A; EC 3.2.1.22; Melibiase; Alpha-D-Galactoside Galactohydrolase 1; Alpha-D-Galactoside Galactohydrolase; Agalsidase Alfa; Alpha-Gal A; Agalsidase; EC 3.2.1
Background	Gene Name: GLA NCBI Gene Entry: 2717 UniProt Entry: P06280
Application Information	Molecular Weight: Predicted, 49 kDa; observed, 46 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11325 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Galactosidase alpha
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of galactosidase alpha expression in Hela cells using anti-galactosidase alpha antibody (Cat#4459, 1:2,000). Green, isotype control; red, galactosidase alpha.
Immunocytochemical staining of Hela cells with anti-Galactosidase alpha antibody (Cat#4459, 1:1,000). Nuclei were stained blue with DAPI; Galactosidase alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-galactosidase alpha antibody (Cat#4459). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-galactosidase alpha antibody (Cat#4459, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

GLA
货号：

4459
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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