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Anti-PFKM Rabbit Monoclonal Antibody #4465

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Flow cytometric analysis of PFKM expression in HepG2 cells using anti-PFKM antibody (Cat#4465, 1:2,000). Green, isotype control; red, PFKM.

Immunocytochemical staining of HepG2 cells with anti-PFKM antibody (Cat#4465, 1:1,000). Nuclei were stained blue with DAPI;
PFKM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PFKM antibody (Cat#4465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKM antibody (Cat#4465, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PFKM
货号：

4465
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-PFKM Rabbit Monoclonal Antibody
Aliases	PFKM; Phosphofructokinase, Muscle; PPP1R122; PFK-1; PFKX; ATP-Dependent 6-Phosphofructokinase, Muscle Type; Protein Phosphatase 1, Regulatory Subunit 122; Phosphofructokinase, Polypeptide X; Phosphofructo-1-Kinase Isozyme A; 6-Phosphofructokinase Type A; Phosphohexokinase; EC 2.7.1.11; ATP-PFK; PFK-A; 6-Phosphofructokinase, Muscle Type; 6-Phosphofructo-1-Kinase; Phosphofructokinase 1; Phosphofructokinase-M; EC 2.7.1; PFK-M; GSD7; PFK1; PFKA
Background	Gene Name: PFKM NCBI Gene Entry: 5213 UniProt Entry: P08237
Application Information	Molecular Weight: Predicted, 85 kDa; observed, 75 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11355 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Fructose 6 Phosphate Kinase
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PFKM expression in HepG2 cells using anti-PFKM antibody (Cat#4465, 1:2,000). Green, isotype control; red, PFKM.
Immunocytochemical staining of HepG2 cells with anti-PFKM antibody (Cat#4465, 1:1,000). Nuclei were stained blue with DAPI;
PFKM was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PFKM antibody (Cat#4465). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKM antibody (Cat#4465, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PFKM
货号：

4465
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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