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Anti-AKR1C1/AKR1C2 Rabbit Monoclonal Antibody #4469

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Flow cytometric analysis of AKR1C1/AKR1C2 expression in HeLa cells using anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:2,000). Green, isotype control; red, AKR1C1/AKR1C2.
Immunocytochemical staining of Hela cells with anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:1,000). Nuclei were stained blue with DAPI; AKR1C1/AKR1C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-AKR1C1/AKR1C2 antibody (Cat#4469). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    AKR1C1/AKR1C2
  • 货号:
    4469
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-AKR1C1/AKR1C2 Rabbit Monoclonal Antibody
Aliases AKR1C1/2; Aldo-Keto Reductase Family 1 Member C1/2; DDH; DD1/2; HAKRC; MBAB; DDH1/2; Dihydrodiol Dehydrogenase 1/2; 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase; High-Affinity Hepatic Bile Acid-Binding Protein; Chlordecone Reductase Homolog HAKRC; Dihydrodiol Dehydrogenase 1/2; HBAB; Aldo-Keto Reductase Family 1, Member C1/2 (Dihydrodiol Dehydrogenase 1; 20-Alpha (3-Alpha)-Hydroxysteroid Dehydrogenase); Trans-1,2-Dihydrobenzene-1,2-Diol Dehydrogenase; Type II 3-Alpha-Hydroxysteroid Dehydrogenase; 20 Alpha-Hydroxysteroid Dehydrogenase; 20-Alpha-Hydroxysteroid Dehydrogenase; Hepatic Dihydrodiol Dehydrogenase; Dihydrodiol Dehydrogenase 1/2; Aldo-Keto Reductase C; Indanol Dehydrogenase; 20-ALPHA-HSD; 20-Alpha-HSD; EC 1.1.1.112; EC 1.1.1.209; EC 1.1.1.210; EC 1.1.1.357; EC 1.1.1.149; 2-ALPHA-HSD; EC 1.1.1.51; EC 1.1.1.53; EC 1.1.1.62; EC 1.3.1.20; EC 1.1.1.; EC 1.1.1; DD1/DD2; H-37; C9
Background

Gene Name: AKR1C1/AKR1C2

NCBI Gene Entry: 1645/1646

UniProt Entry: Q04828/P52895

Application Information

Molecular Weight: Predicted, 37 kDa; observed, 35 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB11375

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthetic peptide of human AKR1C1 / AKR1C2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry(FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of AKR1C1/AKR1C2 expression in HeLa cells using anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:2,000). Green, isotype control; red, AKR1C1/AKR1C2.
  • Immunocytochemical staining of Hela cells with anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:1,000). Nuclei were stained blue with DAPI; AKR1C1/AKR1C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-AKR1C1/AKR1C2 antibody (Cat#4469). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of AKR1C1/AKR1C2 expression in HeLa cells using anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:2,000). Green, isotype control; red, AKR1C1/AKR1C2.
Immunocytochemical staining of Hela cells with anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:1,000). Nuclei were stained blue with DAPI; AKR1C1/AKR1C2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-AKR1C1/AKR1C2 antibody (Cat#4469). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AKR1C1/AKR1C2 antibody (Cat#4469, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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