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Anti-DLAT Rabbit Monoclonal Antibody #4482

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Flow cytometric analysis of DLAT expression in HepG2 cells using anti-DLAT antibody (Cat#4482, 1:2,000). Green, isotype control; red, DLAT.

Immunocytochemical staining of HepG2 cells with anti-DLAT Antibody antibody (Cat#4482, 1:1,000) . Nuclei were stained blue with DAPI; DLAT Antibody was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-DLAT antibody (Cat#4482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DLAT antibody (Cat#4482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

DLAT
货号：

4482
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-DLAT Rabbit Monoclonal Antibody
Aliases	DLAT; Dihydrolipoamide S-Acetyltransferase; PDC-E2; DLTA; E2; Dihydrolipoyllysine-Residue Acetyltransferase Component Of Pyruvate; Dehydrogenase Complex, Mitochondrial; Dihydrolipoamide Acetyltransferase Component Of Pyruvate Dehydrogenase Complex; 70 KDa Mitochondrial Autoantigen Of Primary Biliary Cirrhosis; E2 Component Of Pyruvate Dehydrogenase Complex; Pyruvate Dehydrogenase Complex Component E2; M2 Antigen Complex 70 KDa Subunit; EC 2.3.1.12; PDCE2; PBC; Dihydrolipoyllysine-Residue Acetyltransferase; EC 2.3.1
Background	Gene Name: DLAT NCBI Gene Entry: 1737 UniProt Entry: P10515
Application Information	Molecular Weight: Predicted, 71 kDa; observed, 71 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11440 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human DLAT
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of DLAT expression in HepG2 cells using anti-DLAT antibody (Cat#4482, 1:2,000). Green, isotype control; red, DLAT.
Immunocytochemical staining of HepG2 cells with anti-DLAT Antibody antibody (Cat#4482, 1:1,000) . Nuclei were stained blue with DAPI; DLAT Antibody was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-DLAT antibody (Cat#4482). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DLAT antibody (Cat#4482, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

DLAT
货号：

4482
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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