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Anti-ANP32B Rabbit Monoclonal Antibody #4505

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Flow cytometric analysis of ANP32B expression in HepG2 cells using anti-ANP32B antibody (Cat#4505, 1:2,000). Green, isotype control; red, ANP32B.

Immunocytochemical staining of HepG2 cells with anti-ANP32B antibody (Cat#4505, 1:1,000). Nuclei were stained blue with DAPI; ANP32B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.

Western blotting analysis using anti-ANP32B antibody (Cat#4505). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ANP32B antibody (Cat#4505, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

ANP32B
货号：

4505
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-ANP32B Rabbit Monoclonal Antibody
Aliases	ANP32B; Acidic Nuclear Phosphoprotein 32 Family Member B; PHAPI2; APRIL; Acidic Protein Rich In Leucines; SSP29; Acidic (Leucine-Rich) Nuclear Phosphoprotein 32 Family, Member B; Acidic Leucine-Rich Nuclear Phosphoprotein 32 Family Member B; Putative HLA-DR-Associated Protein I-2; Silver-Stainable Protein SSP29
Background	Gene Name: ANP32B NCBI Gene Entry: 10541 UniProt Entry: Q92688
Application Information	Molecular Weight: Predicted, 29 kDa; observed, 25 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11555 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PHAPI2 / APRIL
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ANP32B expression in HepG2 cells using anti-ANP32B antibody (Cat#4505, 1:2,000). Green, isotype control; red, ANP32B.
Immunocytochemical staining of HepG2 cells with anti-ANP32B antibody (Cat#4505, 1:1,000). Nuclei were stained blue with DAPI; ANP32B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-ANP32B antibody (Cat#4505). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ANP32B antibody (Cat#4505, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

ANP32B
货号：

4505
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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