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Anti-Lamin B1 Rabbit Monoclonal Antibody #4551

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Flow cytometric analysis of Lamin B1 expression in C2C12 cells using Lamin B1 antibody (Cat#4551,1:2,000). Green, isotype control; red, Lamin B1.
Immunocytochemical staining of HepG2 cells with anti-Lamin B1 antibody (Cat#4551, 1:1,000). Nuclei were stained blue with DAPI; Lamin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Lamin B1 antibody (Cat#4551). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lamin B1 antibody (Cat#4551,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    LMNB1
  • 货号:
    4551
  • 应用:
    WB
  • 物种反应性:
    H, M, R
规格:
50 μL,100 μL
价格:
¥ 600.00,¥ 1,000.00
Information
Product Name Anti-Lamin B1 Rabbit Monoclonal Antibody
Aliases LMNB1; Lamin B1; Lamin-B1; LMN2; LMNB; MCPH26; ADLD; LMN
Background

Gene Name: LMNB1

NCBI Gene Entry: 4001

UniProt Entry: P20700

Application Information

Molecular Weight: Predicted, 66 kDa; observed, 70 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB915

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Lamin B1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Lamin B1 expression in C2C12 cells using Lamin B1 antibody (Cat#4551,1:2,000). Green, isotype control; red, Lamin B1.
  • Immunocytochemical staining of HepG2 cells with anti-Lamin B1 antibody (Cat#4551, 1:1,000). Nuclei were stained blue with DAPI; Lamin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
  • Western blotting analysis using anti-Lamin B1 antibody (Cat#4551). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lamin B1 antibody (Cat#4551,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Lamin B1 expression in C2C12 cells using Lamin B1 antibody (Cat#4551,1:2,000). Green, isotype control; red, Lamin B1.
Immunocytochemical staining of HepG2 cells with anti-Lamin B1 antibody (Cat#4551, 1:1,000). Nuclei were stained blue with DAPI; Lamin B1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar: 20 μm.
Western blotting analysis using anti-Lamin B1 antibody (Cat#4551). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Lamin B1 antibody (Cat#4551,1:10,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201,1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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