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Anti-ATP7B Rabbit Monoclonal Antibody #4579

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Flow cytometric analysis of ATP7B expression in HepG2 cells using anti-ATP7B antibody (Cat#4579, 1:2,000). Green, isotype control; red, ATP7B.

Immunocytochemical staining of HepG2 cells with anti-ATP7B antibody (Cat#4579, 1:1,000). Nuclei were stained blue with DAPI; ATP7B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-ATP7B antibody (Cat#4579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP7B antibody (Cat#4579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

ATP7B
货号：

4579
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-ATP7B Rabbit Monoclonal Antibody
Aliases	ATP7B; ATPase Copper Transporting Beta; Copper-Transporting ATPase 2; Copper Pump 2; WND; ATPase, Cu++ Transporting, Beta Polypeptide; Wilson Disease-Associated Protein; PWD; WC1; ATPase, Cu++ Transporting, Beta Polypeptide (Wilson Disease); ATPase, Cu(2+)- Transporting, Beta Polypeptide; Copper-Transporting Protein ATP7B; Wilson Disease; EC 7.2.2.8; EC 3.6.3.4; EC 3.6.3; WD
Background	Gene Name: ATP7B NCBI Gene Entry: 540 UniProt Entry: P35670
Application Information	Molecular Weight: Predicted, 157 kDa; observed, 157 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11915 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human ATP7b
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of ATP7B expression in HepG2 cells using anti-ATP7B antibody (Cat#4579, 1:2,000). Green, isotype control; red, ATP7B.
Immunocytochemical staining of HepG2 cells with anti-ATP7B antibody (Cat#4579, 1:1,000). Nuclei were stained blue with DAPI; ATP7B was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-ATP7B antibody (Cat#4579). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ATP7B antibody (Cat#4579, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

ATP7B
货号：

4579
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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