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Anti-DNA Polymerase gamma Rabbit Monoclonal Antibody #4590

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Flow cytometric analysis of POLG expression in HeLa cells using anti-POLG antibody (Cat#4590, 1:2,000). Green, isotype control; red, POLG.

Immunocytochemical staining of HeLa cells with anti-POLG antibody (Cat#4590, 1:1,000). Nuclei were stained blue with DAPI; POLG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-POLG antibody (Cat#4590). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POLG antibody (Cat#4590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

POLG
货号：

4590
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information

Product Name	Anti-DNA Polymerase gamma Rabbit Monoclonal Antibody
Aliases	POLG; DNA Polymerase Gamma, Catalytic Subunit; POLG1; POLGA; Mitochondrial DNA Polymerase Catalytic Subunit; Polymerase (DNA) Gamma, Catalytic Subunit; Polymerase (DNA Directed), Gamma; DNA Polymerase Subunit Gamma-1; 5'-Deoxyribose-Phosphate Lyase; 3'-5' Exodeoxyribonuclease; PolG-Alpha; EC 2.7.7.7; MDP1; Mitochondrial Polymerase Gamma Catalytic Subunit; EC 3.1.11.-; EC 4.2.99.-; MTDPS4A; MTDPS4B; MIRAS; SANDO; SCAE; PEO
Background	Gene Name: POLG NCBI Gene Entry: 5428 UniProt Entry: P54098
Application Information	Molecular Weight: Predicted, 140 kDa; observed, 140 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB11965 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human POLG
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000 Flow Cytometry (FCM): 1:2,00
Note	This product is for research use only.

Picture

Flow cytometric analysis of POLG expression in HeLa cells using anti-POLG antibody (Cat#4590, 1:2,000). Green, isotype control; red, POLG.
Immunocytochemical staining of HeLa cells with anti-POLG antibody (Cat#4590, 1:1,000). Nuclei were stained blue with DAPI; POLG was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-POLG antibody (Cat#4590). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-POLG antibody (Cat#4590, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

POLG
货号：

4590
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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