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Anti-Syndecan binding protein Rabbit Monoclonal Antibody #4599

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Flow cytometric analysis of syndecan binding protein expression in HepG2 cells using anti-syndecan binding protein antibody (Cat#4599, 1:2,000). Green, isotype control; red, syndecan binding protein.
Immunocytochemical staining of HepG2 cells with anti-Syndecan binding protein antibody (Cat#4599, 1:1,000). Nuclei were stained blue with DAPI; Syndecan binding protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-syndecan binding protein antibody (Cat#4599). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-syndecan binding protein antibody (Cat#4599, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    SDCBP
  • 货号:
    4599
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Syndecan binding protein Rabbit Monoclonal Antibody
Aliases SDCBP; Syndecan Binding Protein; MDA-9; SYCL; Syntenin-1; Pro-TGF-Alpha Cytoplasmic Domain-Interacting Protein 18; Syndecan-Binding Protein 1; Scaffold Protein Pbp1; TACIP18; SDCBP1; MDA9; Melanoma Differentiation Associated Protein-9; Melanoma Differentiation-Associated Protein 9; Syndecan Binding Protein (Syntenin); Syntenin; ST1
Background

Gene Name: SDCBP

NCBI Gene Entry: 6386

UniProt Entry: O00560

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 32 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12010

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human Syntenin
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of syndecan binding protein expression in HepG2 cells using anti-syndecan binding protein antibody (Cat#4599, 1:2,000). Green, isotype control; red, syndecan binding protein.
  • Immunocytochemical staining of HepG2 cells with anti-Syndecan binding protein antibody (Cat#4599, 1:1,000). Nuclei were stained blue with DAPI; Syndecan binding protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-syndecan binding protein antibody (Cat#4599). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-syndecan binding protein antibody (Cat#4599, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of syndecan binding protein expression in HepG2 cells using anti-syndecan binding protein antibody (Cat#4599, 1:2,000). Green, isotype control; red, syndecan binding protein.
Immunocytochemical staining of HepG2 cells with anti-Syndecan binding protein antibody (Cat#4599, 1:1,000). Nuclei were stained blue with DAPI; Syndecan binding protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-syndecan binding protein antibody (Cat#4599). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-syndecan binding protein antibody (Cat#4599, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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