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Anti-Calpain small subunit 1 Rabbit Monoclonal Antibody #4637

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Flow cytometric analysis of Calpain small subunit 1 expression in HepG2 cells using anti-Calpain small subunit 1 antibody (Cat#4637, 1:2,000). Green, isotype control; red, Calpain small subunit 1.

Immunocytochemical staining of HepG2 cells with anti-Calpain small subunit 1 antibody (Cat#4637, 1:1,000) . Nuclei were stained blue with DAPI; Calpain small subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-calpain small subunit 1 antibody (Cat#4637). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calpain small subunit 1 antibody (Cat#4637, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

CAPNS1
货号：

4637
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

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Information

Product Name	Anti-Calpain small subunit 1 Rabbit Monoclonal Antibody
Aliases	CAPNS1; Calpain Small Subunit 1; CDPS; CANPS; CAPN4; CANP; Calcium-Activated Neutral Proteinase Small Subunit; Calcium-Dependent Protease Small Subunit 1; Calpain Regulatory Subunit; CANP Small Subunit; CSS1; 30K; Epididymis Secretory Sperm Binding Protein; Calcium-Dependent Protease, Small Subunit; Calcium-Dependent Protease Small Subunit; Calpain 4, Small Subunit (30K); Calpain, Small Polypeptide; CALPAIN4; CAPNS
Background	Gene Name: CAPNS1 NCBI Gene Entry: 826 UniProt Entry: P04632
Application Information	Molecular Weight: Predicted, 28 kDa; observed, 28 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12195 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human Calpain small subunit 1
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Calpain small subunit 1 expression in HepG2 cells using anti-Calpain small subunit 1 antibody (Cat#4637, 1:2,000). Green, isotype control; red, Calpain small subunit 1.
Immunocytochemical staining of HepG2 cells with anti-Calpain small subunit 1 antibody (Cat#4637, 1:1,000) . Nuclei were stained blue with DAPI; Calpain small subunit 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-calpain small subunit 1 antibody (Cat#4637). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-calpain small subunit 1 antibody (Cat#4637, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

CAPNS1
货号：

4637
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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