Flow cytometric analysis of Histone H3 (acetyl K27) expression in HeLa cells using anti-Histone H3 (acetyl K27) antibody (Cat#4642, 1:2,000). Green, isotype control; red, Histone H3 (acetyl K27).
Immunocytochemical staining of HeLa cells with anti-Histone H3 (acetyl K27) antibody (Cat#4642, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (acetyl K27) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-histone H3 (acetyl K28) antibody (Cat#4642). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-histone H3 (acetyl K28) antibody (Cat#4642, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-histone H3 (acetyl K28) antibody (Cat#4642, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
