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Anti-PRKAR2A Rabbit Monoclonal Antibody #4646

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Flow cytometric analysis of PRKAR2A expression in HAP-1 cells using anti-PRKAR2A antibody (Cat#4646, 1:2,000). Green, isotype control; red, PRKAR2A.

Immunocytochemical staining of HAP1 cells with anti-PRKAR2A antibody (Cat#4646, 1:1,000) . Nuclei were stained blue with DAPI;PRKAR2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PRKAR2A antibody (Cat#4646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PRKAR2A antibody (Cat#4646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-PRKAR2A antibody (Cat#4646, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

基因名：

PRKAR2A
货号：

4646
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Citation(0)

Information

Product Name	Anti-PRKAR2A Rabbit Monoclonal Antibody
Aliases	Protein Kinase CAMP-Dependent Type II Regulatory Subunit Alpha; PRKAR2; Protein Kinase, CAMP-Dependent, Regulatory Subunit Type II Alpha; CAMP-Dependent Protein Kinase Type II-Alpha Regulatory Subunit; PKR2; Protein Kinase, CAMP-Dependent, Regulatory, Type II, Alpha; CAMP-Dependent Protein Kinase Regulatory Subunit RII Alpha; Protein Kinase A, RII-Alpha Subunit
Background	Gene Name: PRKAR2A NCBI Gene Entry: 5576 UniProt Entry: P13861
Application Information	Molecular Weight: Predicted, 46 kDa; observed, 52 kDa Clonality: Rabbit monoclonal antibody Clone ID: 24GB12240 Species Reactivity: Human, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	A synthesized peptide derived from human PKA R2
Isotype	Rabbit IgG
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:1,000-1:5,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PRKAR2A expression in HAP-1 cells using anti-PRKAR2A antibody (Cat#4646, 1:2,000). Green, isotype control; red, PRKAR2A.
Immunocytochemical staining of HAP1 cells with anti-PRKAR2A antibody (Cat#4646, 1:1,000) . Nuclei were stained blue with DAPI;PRKAR2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PRKAR2A antibody (Cat#4646). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PRKAR2A antibody (Cat#4646, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human sigmoid colon carcinoma using anti-PRKAR2A antibody (Cat#4646, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.

Citation(0)

基因名：

PRKAR2A
货号：

4646
应用：

WB, FCM, IC
物种反应性：

H, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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