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Anti-Linker For Activation Of T Cells Rabbit Monoclonal Antibody #4723

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Flow cytometric analysis of LAT expression in HT-1080 cells using anti-LAT antibody (Cat#4723, 1:2,000). Green, isotype control; red, LAT.
Immunocytochemical staining of HT-1080 cells with anti-LAT antibody (Cat#4723, 1:1,000) . Nuclei were stained blue with DAPI; LAT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-LAT antibody (Cat#4723). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LAT antibody (Cat#4723, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    LAT
  • 货号:
    4723
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Linker For Activation Of T Cells Rabbit Monoclonal Antibody
Aliases LAT; Linker For Activation Of T Cells; LAT1; Linker For Activation Of T Cells,Transmembrane Adaptor; Linker For Activation Of T-Cells Family Member 1; 36 KDa Phosphotyrosine Adapter Protein; P36-38; Pp36; 36 KDa Phospho-Tyrosine Adapter Protein; 36 KDa Phospho-Tyrosine Adaptor Protein; IMD52
Background

Gene Name: LAT

NCBI Gene Entry: 27040

UniProt Entry: O43561

Application Information

Molecular Weight: Predicted, 28 kDa; observed, 36 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB12625

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human LAT
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of LAT expression in HT-1080 cells using anti-LAT antibody (Cat#4723, 1:2,000). Green, isotype control; red, LAT.
  • Immunocytochemical staining of HT-1080 cells with anti-LAT antibody (Cat#4723, 1:1,000) . Nuclei were stained blue with DAPI; LAT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-LAT antibody (Cat#4723). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LAT antibody (Cat#4723, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of LAT expression in HT-1080 cells using anti-LAT antibody (Cat#4723, 1:2,000). Green, isotype control; red, LAT.
Immunocytochemical staining of HT-1080 cells with anti-LAT antibody (Cat#4723, 1:1,000) . Nuclei were stained blue with DAPI; LAT was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-LAT antibody (Cat#4723). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-LAT antibody (Cat#4723, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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