Flow cytometric analysis of Phospho-PKC alpha (Ser657) expression in HT-1080 cells using anti-Phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:2,000). Green, isotype control; red, Phospho-PKC alpha (Ser657).
Immunocytochemical staining of HT-1080 cells with anti-PRKCA antibody (Cat#4751, 1:1,000) . Nuclei were stained blue with DAPI; PRKCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-PKC alpha (Ser657) antibody (Cat#4751). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-PKC alpha (Ser657) expression in HT-1080 cells using anti-Phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:2,000). Green, isotype control; red, Phospho-PKC alpha (Ser657).
Immunocytochemical staining of HT-1080 cells with anti-PRKCA antibody (Cat#4751, 1:1,000) . Nuclei were stained blue with DAPI; PRKCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-PKC alpha (Ser657) antibody (Cat#4751). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Flow cytometric analysis of Phospho-PKC alpha (Ser657) expression in HT-1080 cells using anti-Phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:2,000). Green, isotype control; red, Phospho-PKC alpha (Ser657).
Immunocytochemical staining of HT-1080 cells with anti-PRKCA antibody (Cat#4751, 1:1,000) . Nuclei were stained blue with DAPI; PRKCA was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-phospho-PKC alpha (Ser657) antibody (Cat#4751). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-PKC alpha (Ser657) antibody (Cat#4751, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
