Anti-Epidermal Growth Factor Receptor Mouse Monoclonal Antibody - 产品中心

Anti-Epidermal Growth Factor Receptor Mouse Monoclonal Antibody #4796

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Flow cytometric analysis of Epidermal growth factor receptor expression in HepG2 cells using anti-Epidermal growth factor receptor antibody (Cat#4796, 1:2,000). Green, isotype control; red, Epidermal growth factor receptor.

Immunocytochemical staining of HepG2 cells with anti-Epidermal growth factor receptor antibody (Cat#4796, 1:1,000) . Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-epidermal growth factor receptor antibody (Cat#4796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epidermal growth factor receptor antibody (Cat#4796, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

EGFR
货号：

4796
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Citation(0)

Information

Product Name	Anti-Epidermal Growth Factor Receptor Mouse Monoclonal Antibody
Aliases	EGFR; Epidermal Growth Factor Receptor; ERBB1; ERRP; ERBB; Receptor Tyrosine-Protein Kinase ErbB-1; Erb-B2 Receptor Tyrosine Kinase 1; Proto-Oncogene C-ErbB-1; EC 2.7.10.1; HER1; Epidermal Growth Factor Receptor (Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog); Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian); Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog; Epidermal Growth Factor Receptor Tyrosine Kinase Domain; Cell Proliferation-Inducing Protein 61; Cell Growth Inhibiting Protein 40; EGFR VIII; EC 2.7.10; NISBD2; PIG61; MENA
Background	Gene Name: EGFR NCBI Gene Entry: 1956 UniProt Entry: P00533
Application Information	Molecular Weight: Predicted, 134 kDa; observed, 170 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB12985 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human EGFR
Isotype	Mouse IgG2b
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB):1:500-1:2,500 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of Epidermal growth factor receptor expression in HepG2 cells using anti-Epidermal growth factor receptor antibody (Cat#4796, 1:2,000). Green, isotype control; red, Epidermal growth factor receptor.
Immunocytochemical staining of HepG2 cells with anti-Epidermal growth factor receptor antibody (Cat#4796, 1:1,000) . Nuclei were stained blue with DAPI; Epidermal growth factor receptor was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-epidermal growth factor receptor antibody (Cat#4796). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epidermal growth factor receptor antibody (Cat#4796, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

EGFR
货号：

4796
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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