Flow cytometric analysis of HDGF expression in HeLa cells using anti-HDGF antibody (Cat#4822, 1:2,000). Green, isotype control; red, HDGF.
Western blotting analysis using anti-HDGF antibody (Cat#4822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HDGF antibody (Cat#4822, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Applications Tested: Western blotting (WB), flow cytometry (FCM)
Immunogen
Recombinant protein of human HDGF
Isotype
Mouse IgG1
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000
Note
This product is for research use only.
Picture
Flow cytometric analysis of HDGF expression in HeLa cells using anti-HDGF antibody (Cat#4822, 1:2,000). Green, isotype control; red, HDGF.
Western blotting analysis using anti-HDGF antibody (Cat#4822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HDGF antibody (Cat#4822, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
Flow cytometric analysis of HDGF expression in HeLa cells using anti-HDGF antibody (Cat#4822, 1:2,000). Green, isotype control; red, HDGF.
Western blotting analysis using anti-HDGF antibody (Cat#4822). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HDGF antibody (Cat#4822, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat#226).
