Flow cytometric analysis of WTAP expression in HeLa cells using anti-WTAP antibody (Cat#4869, 1：2000). Green, isotype control; red, WTAP.
Immunocytochemical staining of Hela cells with anti-WTAP associated protein antibody (Cat#4869, 1:1000) . Nuclei were stained blue with DAPI; WTAP associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. Permeabilization: Triton.
Western blotting analysis using anti-WTAP antibody (Cat #4869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WTAP antibody (Cat #4869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat #226).
Flow cytometric analysis of WTAP expression in HeLa cells using anti-WTAP antibody (Cat#4869, 1：2000). Green, isotype control; red, WTAP.
Immunocytochemical staining of Hela cells with anti-WTAP associated protein antibody (Cat#4869, 1:1000) . Nuclei were stained blue with DAPI; WTAP associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. Permeabilization: Triton.
Western blotting analysis using anti-WTAP antibody (Cat #4869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WTAP antibody (Cat #4869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat #226).
Flow cytometric analysis of WTAP expression in HeLa cells using anti-WTAP antibody (Cat#4869, 1：2000). Green, isotype control; red, WTAP.
Immunocytochemical staining of Hela cells with anti-WTAP associated protein antibody (Cat#4869, 1:1000) . Nuclei were stained blue with DAPI; WTAP associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. Permeabilization: Triton.
Western blotting analysis using anti-WTAP antibody (Cat #4869). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-WTAP antibody (Cat #4869, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using FeQTM ECL Substrate Kit (Cat #226).
