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Anti-DDX21 Rabbit Monoclonal Antibody #4891

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Flow cytometric analysis of DDX21 expression in HAP-1 cells using anti-DDX21 antibody (Cat#4891, 1:2,000). Green, isotype control; red, DDX21.
Immunocytochemical staining of HAP-1 cells with anti-DDX21 antibody (Cat#4891, 1:1,000) . Nuclei were stained blue with DAPI; DDX21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
Western blotting analysis using anti-DDX21 antibody (Cat#4891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDX21 antibody (Cat#4891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    DDX21
  • 货号：
    4891
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-DDX21 Rabbit Monoclonal Antibody
Aliases DDX21; DExD-Box Helicase 21; Gu-Alpha; Nucleolar RNA Helicase 2; RH-II/GU; GURDB; DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21; Nucleolar RNA Helicase Gu; Nucleolar RNA Helicase II; DEAD-Box Helicase 21; DEAD Box Protein 21; RH II/Gu; DEAD (Asp-Glu-Ala-Asp) Box Helicase 21; RNA Helicase II/Gu Alpha; EC 3.6.4.13; Gu Protein; RH-II/GuA; EC 3.6.1 47; II/Gu; GUA; RH
Background

Gene Name: DDX21

NCBI Gene Entry: 9188

UniProt Entry: Q9NR30
Application Information

Molecular Weight: Predicted, 87 kDa; observed, 87 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB13615

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC), flow cytometry (FCM)
Immunogen A synthesized peptide derived from human DDX21
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Flow Cytometry (FCM) : 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of DDX21 expression in HAP-1 cells using anti-DDX21 antibody (Cat#4891, 1:2,000). Green, isotype control; red, DDX21.
  • Immunocytochemical staining of HAP-1 cells with anti-DDX21 antibody (Cat#4891, 1:1,000) . Nuclei were stained blue with DAPI; DDX21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
  • Western blotting analysis using anti-DDX21 antibody (Cat#4891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDX21 antibody (Cat#4891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    DDX21
  • 货号：
    4891
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Flow cytometric analysis of DDX21 expression in HAP-1 cells using anti-DDX21 antibody (Cat#4891, 1:2,000). Green, isotype control; red, DDX21.
Immunocytochemical staining of HAP-1 cells with anti-DDX21 antibody (Cat#4891, 1:1,000) . Nuclei were stained blue with DAPI; DDX21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:Medium . Scale bar, 20 μm.
Western blotting analysis using anti-DDX21 antibody (Cat#4891). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DDX21 antibody (Cat#4891, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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