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Anti-Gamma-actin-1 Rabbit Polyclonal Antibody #50134

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Immunohistochemical analysis of Gamma-actin-1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Gamma-actin-1 expression in HEK293T (A), Hela (B), mouse brain (C), rat muscle (D), rat brain (E), rat spleen (F) whole cell lysates.
  • 基因名:
    ACTG1
  • 货号:
    50134
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, R, B, C, P, Rb, S
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-Gamma-actin-1 Rabbit Polyclonal Antibody
Aliases ACTB; Actin, cytoplasmic 1; Beta-actin; POTEKP; ACTBL3; FKSG30; Putative beta-actin-like protein 3; Kappa-actin; POTE ankyrin domain family member K; ACTG1; ACTB; ACTG; Actin, cytoplasmic 2; Gamma-actin
Background

Gene Name: ACTG1

NCBI Gene Entry: 60/71

UniProt Entry: P60709/Q9BYX7/P63261

Application Information

Molecular Weight: Predicted, 41,42 kDa; observed, 42 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, pig, rabbit, sheep

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human Gamma-actin-1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of Gamma-actin-1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of Gamma-actin-1 expression in HEK293T (A), Hela (B), mouse brain (C), rat muscle (D), rat brain (E), rat spleen (F) whole cell lysates.
Citation(0)
Immunohistochemical analysis of Gamma-actin-1 staining in human muscle formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Gamma-actin-1 expression in HEK293T (A), Hela (B), mouse brain (C), rat muscle (D), rat brain (E), rat spleen (F) whole cell lysates.
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