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Anti-Connexin 32 Rabbit Polyclonal Antibody #50348

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Immunohistochemical analysis of Connexin 32 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Connexin 32 expression in LOVO (A), mouse brain (B), mouse lung (C), mouse kidney (D), rat kidney (E) whole cell lysates.
  • 基因名:
    GJB1
  • 货号:
    50348
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, R, B, D, P, Mk
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-Connexin 32 Rabbit Polyclonal Antibody
Aliases CX32; Gap junction beta-1 protein; Connexin-32; Cx32; GAP junction 28 kDa liver protein
Background

Gene Name: GJB1

NCBI Gene Entry: 2705

UniProt Entry: P08034

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 28 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, dog, pig, monkey

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human Connexin 32
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of Connexin 32 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of Connexin 32 expression in LOVO (A), mouse brain (B), mouse lung (C), mouse kidney (D), rat kidney (E) whole cell lysates.
Citation(0)
Immunohistochemical analysis of Connexin 32 staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. AEC was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Connexin 32 expression in LOVO (A), mouse brain (B), mouse lung (C), mouse kidney (D), rat kidney (E) whole cell lysates.
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