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Anti-NRAS/HRAS/KRAS Rabbit Polyclonal Antibody #50391

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Immunocytochemical analysis of NRAS/HRAS/KRAS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of NRAS/HRAS/KRAS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of NRAS/HRAS/KRAS expression in H446 (A), mouse kidney (B), mouse testis (C), rat kidney (D) whole cell lysates.
  • 基因名:
    NRAS/HRAS/KRAS
  • 货号:
    50391
  • 应用:
    WB, IHC, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-NRAS/HRAS/KRAS Rabbit Polyclonal Antibody
Aliases NRAS; HRAS1; GTPase NRas; Transforming protein N-Ras; HRAS; HRAS1; GTPase HRas; H-Ras-1; Ha-Ras; Transforming protein p21; c-H-ras; p21ras; KRAS; KRAS2; RASK2; GTPase KRas; K-Ras 2; Ki-Ras; c-K-ras; c-Ki-ras
Background

Gene Name: NRAS/HRAS/KRAS

NCBI Gene Entry: 3265/3845/4893

UniProt Entry: P01111/P01112/P01116

Application Information

Molecular Weight: Predicted, 21 kDa; observed, 21 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human NRAS/HRAS/KRAS
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:50-1:100
Immunocytochemistry (IC): 1:50-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of NRAS/HRAS/KRAS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemical analysis of NRAS/HRAS/KRAS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of NRAS/HRAS/KRAS expression in H446 (A), mouse kidney (B), mouse testis (C), rat kidney (D) whole cell lysates.
Citation(0)
Immunocytochemical analysis of NRAS/HRAS/KRAS staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of NRAS/HRAS/KRAS staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of NRAS/HRAS/KRAS expression in H446 (A), mouse kidney (B), mouse testis (C), rat kidney (D) whole cell lysates.
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