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Anti-PTPRC Mouse Monoclonal Antibody #5048

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Western blotting analysis using anti-CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-PTPRC antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名：
    PTPRC
  • 货号：
    5048
  • 应用：
    WB, IHC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

Picture

Citation(0)

Information
Product Name Anti-PTPRC Mouse Monoclonal Antibody
Aliases PTPRC; Protein Tyrosine Phosphatase Receptor Type C; T200; GP180; CD45; LCA; Receptor-Type Tyrosine-Protein Phosphatase C; Glycoprotein 180; CD45 Antigen; CD45R; B220; L-CA; LY5; Protein Tyrosine Phosphatase, Receptor Type, C Polypeptide; T200 Leukocyte Common Antigen; Leukocyte-Common Antigen; Leukocyte Common Antigen; T200 Glycoprotein; EC 3.1.3.48; IMD105
Background

Gene Name: PTPRC

NCBI Gene Entry: 5788

UniProt Entry: P08575
Application Information

Molecular Weight: Predicted, 147 kDa; observed, 180-240 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB14030

Species Reactivity: Human

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen Recombinant protein of human PTPRC
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Immunohistochemistry (IHC): 1:50-1:100
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-PTPRC antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
  • 基因名：
    PTPRC
  • 货号：
    5048
  • 应用：
    WB, IHC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 620.00，¥ 1,200.00，¥ 2,200.00
RELATED PRODUCTS
Western blotting analysis using anti-CD45 antibody (Cat#5048). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD45 antibody (Cat#5048, 1:10,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunohistochemistry was performed on paraffin-embedded human tonsillitis using anti-PTPRC antibody (Cat#5048, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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