Western blotting analysis using anti-UBE2D2 antibody (Cat#50718). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D2 antibody (Cat#50718, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Species Reactivity: Human, mouse, rat, bovine, pig
Applications Tested: Western blotting (WB)
Immunogen
A synthesized peptide derived from human UBE2D2
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-UBE2D2 antibody (Cat#50718). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D2 antibody (Cat#50718, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-UBE2D2 antibody (Cat#50718). Total lysates (30 μg) were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBE2D2 antibody (Cat#50718, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).