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Anti-MRCK alpha Rabbit Polyclonal Antibody #50753

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Immunocytochemical analysis of MRCK alpha staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of MRCK alpha expression in C6 (A), BV2 (B), HEK293T (C), Panc1 (D), U87MG (E) whole cell lysates.
  • 基因名:
    CDC42BPA
  • 货号:
    50753
  • 应用:
    WB, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-MRCK alpha Rabbit Polyclonal Antibody
Aliases KIAA0451; Serine/threonine-protein kinase MRCK alpha; CDC42-binding protein kinase alpha; DMPK-like alpha; Myotonic dystrophy kinase-related CDC42-binding kinase alpha; MRCK alpha; Myotonic dystrophy protein kinase-like alpha
Background

Gene Name: CDC42BPA

NCBI Gene Entry: 8476

UniProt Entry: Q5VT25

Application Information

Molecular Weight: Predicted, 197 kDa; observed, 186 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human MRCK alpha
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:50-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of MRCK alpha staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of MRCK alpha expression in C6 (A), BV2 (B), HEK293T (C), Panc1 (D), U87MG (E) whole cell lysates.
Citation(0)
Immunocytochemical analysis of MRCK alpha staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of MRCK alpha expression in C6 (A), BV2 (B), HEK293T (C), Panc1 (D), U87MG (E) whole cell lysates.
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