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Anti-MDA5 Rabbit Polyclonal Antibody #50930

一键复制产品信息
Immunohistochemical analysis of MDA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of MDA5 expression in A549 (A) whole cell lysates.
  • 基因名:
    IFIH1
  • 货号:
    50930
  • 应用:
    WB, IHC, FCM
  • 物种反应性:
    H, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-MDA5 Rabbit Polyclonal Antibody
Aliases MDA5; RH116; Interferon-induced helicase C domain-containing protein 1; Clinically amyopathic dermatomyositis autoantigen 140 kDa; CADM-140 autoantigen; Helicase with 2 CARD domains; Helicard; Interferon-induced with helicase C domain protein 1; Melanoma differentiation-associated protein 5; MDA-5; Murabutide down-regulated protein; RIG-I-like receptor 2; RLR-2; RNA helicase-DEAD box protein 116
Background

Gene Name: IFIH1

NCBI Gene Entry: 64135

UniProt Entry: Q9BYX4

Application Information

Molecular Weight: Predicted, 116 kDa; observed, 117 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB), immunohistochemistry (IHC), flow cytometry (FCM)

Immunogen A synthesized peptide derived from human MDA5
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:50-1:100
Flow Cytometry (FCM): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of MDA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of MDA5 expression in A549 (A) whole cell lysates.
Citation(0)
Immunohistochemical analysis of MDA5 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of MDA5 expression in A549 (A) whole cell lysates.
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