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Anti-FTO Rabbit Polyclonal Antibody #50940

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Immunocytochemical analysis of FTO staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of FTO expression in HEK293T (A), Hela (B), A549 (C), rat lung (D), rat kidney (E) whole cell lysates.
  • 基因名：
    FTO
  • 货号：
    50940
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, B, D, Mk, P, Rb, S
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-FTO Rabbit Polyclonal Antibody
Aliases KIAA1752; Alpha-ketoglutarate-dependent dioxygenase FTO; Fat mass and obesity-associated protein
Background

Gene Name: FTO

NCBI Gene Entry: 79068

UniProt Entry: Q9C0B1
Application Information

Molecular Weight: Predicted, 6,12,14,58 kDa; observed, 58 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, dog, monkey, pig, rabbit, sheep

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human FTO
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:50-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of FTO staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of FTO expression in HEK293T (A), Hela (B), A549 (C), rat lung (D), rat kidney (E) whole cell lysates.
Citation(0)
  • 基因名：
    FTO
  • 货号：
    50940
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R, B, D, Mk, P, Rb, S
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunocytochemical analysis of FTO staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of FTO expression in HEK293T (A), Hela (B), A549 (C), rat lung (D), rat kidney (E) whole cell lysates.
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