Immunocytochemical analysis of USP30 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of USP30 expression in MCF7 (A), A549 (B), U2OS (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.
Species Reactivity: Human, mouse, rat, dog, monkey
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human USP30
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:1,000 Immunocytochemistry (IC): 1:100-1:500
Note
This product is for research use only.
Picture
Immunocytochemical analysis of USP30 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of USP30 expression in MCF7 (A), A549 (B), U2OS (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.
Immunocytochemical analysis of USP30 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of USP30 expression in MCF7 (A), A549 (B), U2OS (C), mouse liver (D), mouse kidney (E), rat liver (F), rat kidney (G) whole cell lysates.
