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Anti-TRIM65 Mouse Monoclonal Antibody #5128

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Flow cytometric analysis of TRIM65 expression in HepG2 cells using anti-TRIM65 antibody (Cat#5128, 1:2,000) . Green, isotype control; red, TRIM65.

Immunocytochemical staining of HepG2 cells with anti-TRIM65 antibody (Cat#5128, 1:1,000) . Nuclei were stained blue with DAPI; TRIM65 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.

Western blotting analysis using anti-TRIM65 antibody (Cat#5128). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM65 antibody (Cat#5128, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

TRIM65
货号：

5128
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

Information

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Information

Product Name	Anti-TRIM65 Mouse Monoclonal Antibody
Aliases	TRIM65; Tripartite Motif Containing 65; Tripartite Motif-Containing Protein 65; E3 Ubiquitin-Protein Ligase TRIM65; Tripartite Motif-Containing 65; 4732463G12Rik; EC 2.3.2.27
Background	Gene Name: TRIM65 NCBI Gene Entry: 201292 UniProt Entry: Q6PJ69
Application Information	Molecular Weight: Predicted, 57 kDa; observed, 54 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB15855 Species Reactivity: Human Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human TRIM65
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:200-1:1,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of TRIM65 expression in HepG2 cells using anti-TRIM65 antibody (Cat#5128, 1:2,000) . Green, isotype control; red, TRIM65.
Immunocytochemical staining of HepG2 cells with anti-TRIM65 antibody (Cat#5128, 1:1,000) . Nuclei were stained blue with DAPI; TRIM65 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-TRIM65 antibody (Cat#5128). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM65 antibody (Cat#5128, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

TRIM65
货号：

5128
应用：

WB, FCM, IC
物种反应性：

H

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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