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Anti-ATP6V0A2 Rabbit Polyclonal Antibody #51342

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Immunocytochemical analysis of ATP6V0A2 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of ATP6V0A2 expression in mouse brain (A), rat brain (B) whole cell lysates.
  • 基因名:
    ATP6V0A2
  • 货号:
    51342
  • 应用:
    WB, IC
  • 物种反应性:
    H, M, R, Mk
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-ATP6V0A2 Rabbit Polyclonal Antibody
Aliases V-type proton ATPase 116 kDa subunit a isoform 2; V-ATPase 116 kDa isoform a2; Lysosomal H(+)-transporting ATPase V0 subunit a2; TJ6; Vacuolar proton translocating ATPase 116 kDa subunit a isoform 2
Background

Gene Name: ATP6V0A2

NCBI Gene Entry: 23545

UniProt Entry: Q9Y487

Application Information

Molecular Weight: Predicted, 98 kDa; observed, 105 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, monkey

Applications Tested: Western blotting (WB), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human ATP6V0A2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunocytochemistry (IC): 1:50-1:200
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of ATP6V0A2 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
  • Western blotting analysis of ATP6V0A2 expression in mouse brain (A), rat brain (B) whole cell lysates.
Citation(0)
Immunocytochemical analysis of ATP6V0A2 staining in SGC7901 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).
Western blotting analysis of ATP6V0A2 expression in mouse brain (A), rat brain (B) whole cell lysates.
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