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Anti-Claudin 3 Rabbit Polyclonal Antibody #51368

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Immunocytochemical analysis of Claudin 3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Claudin 3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Claudin 3 expression in mouse liver (A), DLD (B), HCT116 (C), PC12 (D) whole cell lysates.
  • 基因名:
    CLDN3
  • 货号:
    51368
  • 应用:
    WB, IHC, IC, IP
  • 物种反应性:
    H, M, R, B
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-Claudin 3 Rabbit Polyclonal Antibody
Aliases C7orf1; CPETR2; Claudin-3; Clostridium perfringens enterotoxin receptor 2; CPE-R 2; CPE-receptor 2; Rat ventral prostate.1 protein homolog; hRVP1
Background

Gene Name: CLDN3

NCBI Gene Entry: 1365

UniProt Entry: O15551

Application Information

Molecular Weight: Predicted, 23 kDa; observed, 23 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine

Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC), immunoprecipitation (IP)

Immunogen A synthesized peptide derived from human Claudin 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Immunocytochemistry (IC): 1:100-1:500
Immunoprecipitation (IP): 1:10-1:100
Note This product is for research use only.
Picture
  • Immunocytochemical analysis of Claudin 3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
  • Immunohistochemical analysis of Claudin 3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of Claudin 3 expression in mouse liver (A), DLD (B), HCT116 (C), PC12 (D) whole cell lysates.
Citation(0)
Immunocytochemical analysis of Claudin 3 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of Claudin 3 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Claudin 3 expression in mouse liver (A), DLD (B), HCT116 (C), PC12 (D) whole cell lysates.
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