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Anti-E2F1 Rabbit Polyclonal Antibody #51383

一键复制产品信息
Immunohistochemical analysis of E2F1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of E2F1 expression in MCF7 (A), A549 (B) whole cell lysates.
  • 基因名:
    E2F1
  • 货号:
    51383
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, R, Mk
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-E2F1 Rabbit Polyclonal Antibody
Aliases RBBP3; Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1
Background

Gene Name: E2F1

NCBI Gene Entry: 1869

UniProt Entry: Q01094

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 70 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, monkey

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human E2F1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of E2F1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of E2F1 expression in MCF7 (A), A549 (B) whole cell lysates.
Citation(0)
Immunohistochemical analysis of E2F1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of E2F1 expression in MCF7 (A), A549 (B) whole cell lysates.
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