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Anti-PSMD1 Mouse Monoclonal Antibody #5140

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Flow cytometric analysis of PSMD1 expression in C2C12 cells using anti-PSMD1 antibody (Cat#5140, 1:2,000) . Green, isotype control; red, PSMD1.

Immunocytochemical staining of C2C12 cells with anti-PSMD1 antibody (Cat#5140, 1:1,000) . Nuclei were stained blue with DAPI; PSMD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.

Western blotting analysis using anti-PSMD1 antibody (Cat#5140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMD1 antibody (Cat#5140, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

基因名：

PSMD1
货号：

5140
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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Information

Product Name	Anti-PSMD1 Mouse Monoclonal Antibody
Aliases	PSMD1; Proteasome 26S Subunit, Non-ATPase 1; P112; Rpn2; S1; Proteasome (Prosome, Macropain) 26S Subunit, Non-ATPase, 1; 26S Proteasome Non-ATPase Regulatory Subunit 1; 26S Proteasome Regulatory Subunit RPN2; 26S Proteasome Regulatory Subunit S1; 26S Proteasome Subunit P112
Background	Gene Name: PSMD1 NCBI Gene Entry: 5707 UniProt Entry: Q99460
Application Information	Molecular Weight: Predicted, 106 kDa; observed, 106 kDa Clonality: Mouse monoclonal antibody Clone ID: 24GB15915 Species Reactivity: Human, mouse, rat Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen	Recombinant protein of human PSMD1
Isotype	Mouse IgG1
Storage Buffer	Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage	Store at -20 °C for one year.
Recommended Dilutions	Western Blotting (WB): 1:400-1:2,000 Flow Cytometry (FCM): 1:2,000 Immunocytochemistry (IC): 1:100-1:1,000
Note	This product is for research use only.

Picture

Flow cytometric analysis of PSMD1 expression in C2C12 cells using anti-PSMD1 antibody (Cat#5140, 1:2,000) . Green, isotype control; red, PSMD1.
Immunocytochemical staining of C2C12 cells with anti-PSMD1 antibody (Cat#5140, 1:1,000) . Nuclei were stained blue with DAPI; PSMD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PSMD1 antibody (Cat#5140). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PSMD1 antibody (Cat#5140, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).

Citation(0)

基因名：

PSMD1
货号：

5140
应用：

WB, FCM, IC
物种反应性：

H, M, R

规格：

20 μL，50 μL，100 μL

价格：

¥ 620.00，¥ 1,200.00，¥ 2,200.00

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