Immunocytochemical analysis of HeLa cells, using AurB antibody (Cat#51629). The picture on the right is blocked with the synthesized peptide.
Western blotting analysis using anti-Aurora B antibody (Cat#51629). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aurora B antibody (Cat#51629, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Applications Tested: Western blotting (WB), immunohistochemistry (IHC), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human Aurora B
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:500-1:2,500 Immunohistochemistry (IHC): 1:100-1:300 Immunocytochemistry (IC): 1:200-1:1,000
Note
This product is for research use only.
Picture
Immunocytochemical analysis of HeLa cells, using AurB antibody (Cat#51629). The picture on the right is blocked with the synthesized peptide.
Western blotting analysis using anti-Aurora B antibody (Cat#51629). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aurora B antibody (Cat#51629, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical analysis of HeLa cells, using AurB antibody (Cat#51629). The picture on the right is blocked with the synthesized peptide.
Western blotting analysis using anti-Aurora B antibody (Cat#51629). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Aurora B antibody (Cat#51629, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
