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Anti-Histone H3 (TriMethyl-K27) Rabbit Polyclonal Antibody #52034

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Immunohistochemical analysis of Histone H3 (TriMethyl-K27) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Histone H3 (TriMethyl-K27) expression in SHSY5Y (A), C6 (B), 3T3L1 (C) whole cell lysates.
  • 基因名：
    HIST1H3A/HIST1H3/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B/H3F3C
  • 货号：
    52034
  • 应用：
    WB, IHC
  • 物种反应性：
    H, M, R, B, C, P, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00

Information

Picture

Citation(0)

Information
Product Name Anti-Histone H3 (TriMethyl-K27) Rabbit Polyclonal Antibody
Aliases HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3; H3F3C; Histone H3.3C; Histone H3.5
Background

Gene Name: HIST1H3A/HIST1H3/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B/H3F3C

NCBI Gene Entry: 8350/8351/8352/8353/8354/8355/8356/8357/8358/8968/126961/333932/653604/3020/3021/440093

UniProt Entry: P68431/Q71DI3/P84243/Q6NXT2
Application Information

Molecular Weight: Predicted, 15 kDa; observed, 17 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, pig, zebrafish

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human Histone H3 protein
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:50-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of Histone H3 (TriMethyl-K27) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of Histone H3 (TriMethyl-K27) expression in SHSY5Y (A), C6 (B), 3T3L1 (C) whole cell lysates.
Citation(0)
  • 基因名：
    HIST1H3A/HIST1H3/HIST1H3C/HIST1H3D/HIST1H3E/HIST1H3F/HIST1H3G/HIST1H3H/HIST1H3I/HIST1H3J/HIST2H3A/HIST2H3C/HIST2H3D/H3F3A/H3F3B/H3F3C
  • 货号：
    52034
  • 应用：
    WB, IHC
  • 物种反应性：
    H, M, R, B, C, P, Z
规格：
20 μL，50 μL，100 μL
价格：
¥ 560.00，¥ 1,100.00，¥ 2,000.00
RELATED PRODUCTS
Immunohistochemical analysis of Histone H3 (TriMethyl-K27) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of Histone H3 (TriMethyl-K27) expression in SHSY5Y (A), C6 (B), 3T3L1 (C) whole cell lysates.
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