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Anti-IKK alpha/beta Rabbit Polyclonal Antibody #52079

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Immunohistochemical analysis of IKK alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of IKK alpha/beta expression in HepG2 (A), HEK293T (B), NIH3T3 (C), mouse brain (D), rat heart (E) whole cell lysates.
  • 基因名:
    CHUK/IKBKB
  • 货号:
    52079
  • 应用:
    WB, IHC
  • 物种反应性:
    H, M, R, B, C, Z
规格:
20 μL,50 μL,100 μL
价格:
¥ 560.00,¥ 1,100.00,¥ 2,000.00
Information
Product Name Anti-IKK alpha/beta Rabbit Polyclonal Antibody
Aliases CHUK; IKKA; TCF16; Inhibitor of nuclear factor kappa-B kinase subunit alpha; I-kappa-B kinase alpha; IKK-A; IKK-alpha; IkBKA; IkappaB kinase; Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; IKK1; Nuclear factor NF-kappa-B inhibitor kinase alpha; NFKBIKA; Transcription factor 16; TCF-16; IKBKB; IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB
Background

Gene Name: CHUK/IKBKB

NCBI Gene Entry: 1147/3551

UniProt Entry: O15111/O14920

Application Information

Molecular Weight: Predicted, 84,86 kDa; observed, 85 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, mouse, rat, bovine, chicken, zebrafish

Applications Tested: Western blotting (WB), immunohistochemistry (IHC)

Immunogen A synthesized peptide derived from human IKK alpha/beta
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.3) containing 30% glycerol, and 0.01% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Immunohistochemical analysis of IKK alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
  • Western blotting analysis of IKK alpha/beta expression in HepG2 (A), HEK293T (B), NIH3T3 (C), mouse brain (D), rat heart (E) whole cell lysates.
Citation(0)
Immunohistochemical analysis of IKK alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Western blotting analysis of IKK alpha/beta expression in HepG2 (A), HEK293T (B), NIH3T3 (C), mouse brain (D), rat heart (E) whole cell lysates.
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